Human CD31/PECAM-1 Protein
Catalog No: CD31-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human CD31/PECAM-1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln28-Lys601 (UniProt accession: P16284-1).
Product Details
- Molecular Aliases
- PECAM-1; EndoCAM; PECA1; CD31; FLJ34100; FLJ58394; GPIIA';PECAM1
- Protein Length
- Gln28-Lys601
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 91.2 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD31 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Platelet endothelial cell adhesion molecule 1 (PECAM-1) is an adhesion and signaling receptor that is expressed on endothelial and hematopoietic cells and plays important roles in angiogenesis, vascular permeability, and regulation of cellular responsiveness.
References
- Zhi H, Kanaji T, Fu G, Newman DK, Newman PJ. Generation of PECAM-1 (CD31) conditional knockout mice. Genesis. 2020 Feb;58(2):e23346. doi: 10.1002/dvg.23346. Epub 2019 Nov
- PMID: 31729819; PMCID: PMC7021573.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.