Human Ephrin-B2/EFNB2 Protein
Catalog No: EFNB2-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Ephrin-B2/EFNB2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ile28-Ala229 (accession: NP_004084.1).
Product Details
- Molecular Aliases
- Ephrin B2; EPLG5; HTKL; LERK5; EFNB2
- Protein Length
- Ile28-Ala229
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human EPHB2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA (QC Test).
Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Background
Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
References
- Nakayama A, Nakayama M, Turner CJ, Höing S, Lepore JJ, Adams RH. Ephrin-B2 controls PDGFRβ internalization and signaling. Genes Dev. 2013 Dec 1;27(23):2576-89. doi: 10.1101/gad.224089.113. PMID: 24298057; PMCID: PMC3861671.
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