Mouse Fas/TNFRSF6/CD95 Protein
Catalog No: CD95-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Mouse Fas/TNFRSF6/CD95 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln22-Arg169 (UniProt accession: P25446).
Product Details
- Molecular Aliases
- FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR
- Protein Length
- Gln22-Arg169
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 43.3 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse Fas on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fas is greater than 95% as determined by SEC-HPLC.
Immobilized Human Fas Ligand, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25μg/ml determined by ELISA.
Background
CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
References
- Guégan JP, Legembre P. Nonapoptotic functions of Fas/CD95 in the immune response. FEBS J. 2018 Mar;285(5):809-827. doi: 10.1111/febs.14292. Epub 2017 Nov
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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