Human CXCR5 Nanodisc Protein
Catalog No: CXCR5-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Human CXCR5 Nanodisc Protein is a full-length multi-pass membrane protein presented in a Nanodisc format for antibody discovery, binding assays and method development. Recombinant Human CXCR5 Nanodisc is expressed from HEK293 with His tag at the C-terminus (FITC-equivalent protein is fused on cytoplasmic part). It contains Met1-Phe372 (accession: P32302-1).
Product Details
- Molecular Aliases
- CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1
- Protein Length
- Met1-Phe372
- Expression System
- HEK293
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.
Background
CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it.
References
- MacDonald RJ, Yen A. CXCR5 overexpression in HL-60 cells enhances chemotaxis toward CXCL13 without anticipated interaction partners or enhanced MAPK signaling. In Vitro Cell Dev Biol Anim. 2018 Dec;54(10):725-735. doi: 10.1007/s11626-018-0293-z. Epub 2018 Oct 1. Erratum in: In Vitro Cell Dev Biol Anim. 2020 Oct;56(9):825. doi: 10.1007/s11626-020-00510-0. PMID: 30276608; PMCID: PMC6657802.
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