Human TNFRSF12A/TWEAKR Protein
Catalog No: TNFRSF12A-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TNFRSF12A/TWEAKR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Glu28-Per80 (UniProt accession: Q9NP84-1).
Product Details
- Molecular Aliases
- FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFRSF12A is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNFRSF12A, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA (QC Test).
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Background
Tumor necrosis factor receptor superfamily member 12a (Tnfrsf12a) is a receptor for tumor necrosis factor superfamily member 12 (Tnfsf12). Tnfrsf12a is highly inducible and plays a key role in the development of cardiac hypertrophy and heart failure.
References
- Yerra VG, Batchu SN, Kabir G, Advani SL, Liu Y, Siddiqi FS, Connelly KA, Advani A. Empagliflozin Disrupts a Tnfrsf12a-Mediated Feed Forward Loop That Promotes Left Ventricular Hypertrophy. Cardiovasc Drugs Ther. 2022 Aug;36(4):619-632. doi: 10.1007/s10557-021-07190-2. Epub 2021 Apr 22. PMID: 33886003.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.