Mouse CLEC4A/DCIR Protein
Catalog No: CLEC4A-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Mouse CLEC4A/DCIR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln70-Leu238 (UniProt accession: Q9QZ15).
Product Details
- Molecular Aliases
- CD367; Clec4a2; CLECSF6; DCIR; DDB27; HDCGC13P; LLIR; CLEC4A; DCIRLLIR
- Protein Length
- Gln70-Leu238
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.3 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse CLEC4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CLEC4A is greater than 95% as determined by SEC-HPLC.
Background
Clec4a has been reported to be an immune suppressor of dendritic cells (DCs), but its potential role in cancer therapy remains to be elucidated. silencing of Clec4a2 expression via skin delivery of shRNA produces an effective antitumor response and that Clec4a2 shRNA may have therapeutic potential as an adjuvant for cancer immunotherapy.
References
- Weng TY, Li CJ, Li CY, Hung YH, Yen MC, Chang YW, Chen YH, Chen YL, Hsu HP, Chang JY, Lai MD. Skin Delivery of Clec4a Small Hairpin RNA Elicited an Effective Antitumor Response by Enhancing CD8 Immunity In Vivo. Mol Ther Nucleic Acids. 2017 Dec 15;9:419-427. doi: 10.1016/j.omtn.2017.10.015. Epub 2017 Oct
- PMID: 29246320; PMCID: PMC5701806. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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