Biotinylated Human BTN3A1/CD277 Protein
Catalog No: BTN3A1-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human BTN3A1/CD277 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln30-Gly254 (UniProt accession: O00481-1).
Product Details
- Molecular Aliases
- CD277; BTN3A1; BTF5; BT3.1; BTN3.1
- Protein Length
- Gln30-Gly254
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-Arginine(pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BTN3A1 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human BTN3A1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. (QC Test)
Background
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
References
- Rhodes DA, Chen HC, Price AJ, Keeble AH, Davey MS, James LC, Eberl M, Trowsdale J. Activation of human γδ T cells by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. J Immunol. 2015 Mar 1;194(5):2390-8. doi: 10.4049/jimmunol.1401064. Epub 2015 Jan 30. PMID: 25637025; PMCID: PMC4337483. Copy
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