Biotinylated Human CCL5 Protein
Catalog No: CCL5-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CCL5 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Ser24-Ser91 (UniProt accession: P13501).
Product Details
- Molecular Aliases
- MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
- Protein Length
- Ser24-Ser91
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 10.89 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human CCL5, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Background
The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
References
- Aldinucci D, Colombatti A. The inflammatory chemokine CCL5 and cancer progression. Mediators Inflamm. 2014;2014:292376. doi: 10.1155/2014/292376. Epub 2014 Jan 2. PMID: 24523569; PMCID: PMC3910068.
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