Human Siglec-6/CD327 Protein
Catalog No: CD327-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Siglec-6/CD327 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln27-Val331 (UniProt accession: O43699-3).
Product Details
- Molecular Aliases
- CD327; CD33 antigen-like 1; CD33L1; CDw327; OB-BP1; Siglec-6; SIGLEC6; CD33L; OBBP1
- Protein Length
- Gln27-Val331
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 60.5 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Siglec-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human Siglec-6, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
Background
Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
References
- Macauley M S, Crocker P R, Paulson J C. Siglec-mediated regulation of immune cell function in disease[J]. Nature Reviews Immunology, 2014, 14(10):653-666.
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