Biotinylated Human uPAR/PLAUR Protein
Catalog No: PLAUR-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human uPAR/PLAUR Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu23-Gly305 (UniProt accession: Q03405-1).
Product Details
- Molecular Aliases
- U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
- Protein Length
- Leu23-Gly305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.36 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human uPAR is greater than 95% as determined by SEC-HPLC.
Immobilized Human PLAU, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human uPAR, His Tag with the EC50 of 25.1ng/ml determined by ELISA (QC Test).
Background
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen. Regulation of the activity of u-PA is also mediated by u-PAR.
References
- Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID: 9122717.
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