Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 Protein
Catalog No: ROR1-HB001
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln30-Glu403 (accession: Q01973-1(Human) / A0A2K5WTX7(Cynomolgus) / F6RUP2(Rhesus macaque)).
Product Details
- Molecular Aliases
- ROR1; NTRKR1; dJ537F10.1
- Protein Length
- Gln30-Glu403
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque ROR1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Background
ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members.
References
- Zhang S, Cui B, Lai H, et al. Ovarian cancer stem cells express ROR1, which can be targeted for anti–cancer-stem-cell therapy[J]. Proceedings of the National Academy of Sciences, 2014, 111(48):17266-71.
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