Rhesus macaque IL-18 R1/CD218a Protein
Catalog No: CD218A-CF001
- Species
- Rhesus macaque
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Rhesus macaque IL-18 R1/CD218a Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Cys22-Gly329 (accession: F7H8F9).
Product Details
- Molecular Aliases
- CD218a; IL18Ra; IL18R1; IL1RRP; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL-18Ra;IL1R5; IL18R alpha
- Protein Length
- Cys22-Gly329
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Rhesus macaque IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque IL-18 R1 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque IL-18 R1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-18, No Tag with an affinity constant of 0.71 nM as determined in SPR assay (Biacore T200) (QC Test).
Loaded Rhesus macaque IL-18 R1, hFc Tag on ProA-Biosensor, can bind Human IL-18, No Tag with an affinity constant of 2.01 nM as determined in BLI assay (Gator).
Background
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
References
- Yasuda K, Nakanishi K, Tsutsui H. Interleukin-18 in Health and Disease. Int J Mol Sci. 2019 Feb 2;20(3):649. doi: 10.3390/ijms20030649. PMID: 30717382; PMCID: PMC6387150. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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