Human BMPR1A/ALK-3 Protein
Catalog No: BMPR1A-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human BMPR1A/ALK-3 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln24-Arg152 (UniProt accession: P36894).
Product Details
- Molecular Aliases
- BMPR1A; ALK-3; ALK3; ALK 3
- Protein Length
- Gln24-Arg152
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.13 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human BMPR1A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BMPR1A is greater than 95% as determined by SEC-HPLC.
Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.1-0.4 μg/mL in the presence of 15 ng/mL of recombinant human BMP4. (QC Test)
Background
The type IA bone morphogenetic protein receptor (Bmpr1a), encoded by 11 exons and spanning about 40 kb on chromosome 14 in mice and chromosome 10 in human (Derynck & Feng, 1997; Mishina, Hanks, Miura, Tallquist, & Behringer, 2002), is an essential receptor for BMP signaling.
References
- Jing J, Hinton RJ, Feng JQ. Bmpr1a Signaling in Cartilage Development and Endochondral Bone Formation. Vitam Horm. 2015;99:273-91. doi: 10.1016/bs.vh.2015.06.001. Epub 2015 Jul 15. PMID: 26279380.
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