Mouse TNFSF15 Protein (active trimer)
Catalog No: TNFSF15-MP002
- Species
- Mouse
- Expression System
- HEK293
- Tag
- monomeric Fc
- Activity
- Activity verified
Product overview
Recombinant Mouse TNFSF15 Protein is expressed in HEK293 cells with monomeric Fc tag at the N-terminus. It contains amino acid residues Ala61-Leu252 (UniProt accession: Q5UBV8).
Product Details
- Molecular Aliases
- TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A; VEGI251
- Protein Length
- Ala61-Leu252
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse TNFSF15 is greater than 90% as determined by SEC-HPLC.
The purity of Mouse TNFSF15 is greater than 90% and the molecular weight of this protein is around 140-170 kDa as determined by SEC-MALS.
Immobilized Mouse DR3, His Tag at 0.5μg/ml(100μl/well) on the plate. Dose response curve for Mouse TNFSF15, monomeric Fc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test).
Loaded Mouse TNFSF15, monomeric Fc Tag on ProA-Biosensor, can bind Mouse DR3, His Tag with an affinity constant of 22.80 nM as determined in BLI assay (Gator).
Background
TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins. TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation. Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.
References
- Valatas V, Kolios G, Bamias G. TL1A (TNFSF15) and DR3 (TNFRSF25): A Co-stimulatory System of Cytokines With Diverse Functions in Gut Mucosal Immunity. Front Immunol. 2019 Mar 27;10:583. doi: 10.3389/fimmu.2019.00583. PMID: 30972074; PMCID: PMC6445966.
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