Human TNFSF12/TWEAK Protein
Catalog No: TNFSF12-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TNFSF12/TWEAK Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser43-His249 (UniProt accession: O43508-1).
Product Details
- Molecular Aliases
- APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
- Protein Length
- Ser43-His249
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFSF12 Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA (QC Test).
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Background
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
References
- Wang M, Xie Z, Xu J, Feng Z. TWEAK/Fn14 axis in respiratory diseases. Clin Chim Acta. 2020 Oct;509:139-148. doi: 10.1016/j.cca.2020.06.007. Epub 2020 Jun 8. PMID: 32526219.
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