Biotinylated Human Endoglin/CD105 Protein
Catalog No: CD105-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Endoglin/CD105 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu26-Gly586 (UniProt accession: P17813-1).
Product Details
- Molecular Aliases
- CD105; Endoglin; ENG; ORW; ORW1; END; HHT1
- Protein Length
- Glu26-Gly586
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Endoglin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Endoglin is greater than 85% as determined by SEC-HPLC.
Immobilized Biotinylated Human Endoglin, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA. (QC Test)
Background
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.
References
- Rosen L S, Gordon M S, Robert F, et al. Endoglin for Targeted Cancer Treatment[J]. Current Oncology Reports, 2014, 16(2):365-482.
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