Human SG3/Secretogranin 3 Protein
Catalog No: SG3-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human SG3/Secretogranin 3 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Phe20-Leu468 (UniProt accession: Q8WXD2-1).
Product Details
- Molecular Aliases
- Secretogranin-3; Secretogranin III; SCG3; SGIII; UNQ2502/PRO5990
- Protein Length
- Phe20-Leu468
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 76.93 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Bis-Tris PAGE result.
- Purity
- > 85% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human SG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
Immobilized Human SG3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SCG3 Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA. (QC Test)
Background
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.
References
- Li W, Webster KA, LeBlanc ME, Tian H. Secretogranin III: a diabetic retinopathy-selective angiogenic factor. Cell Mol Life Sci. 2018 Feb;75(4):635-647. doi: 10.1007/s00018-017-2635-5. Epub 2017 Aug 30. PMID: 28856381; PMCID: PMC5771826.
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