Biotinylated Rat GARP&Latent TGF Beta 1 Complex Protein
Catalog No: GARP-RB001
- Species
- Rat
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Rat GARP&Latent TGF Beta 1 Complex Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus of GARP. It contains amino acid residues Ile18-Asn628(GARP) and Leu30-Arg278(Latent TGF Beta 1) (accession: D3ZVD5(GARP) & P17246(Latent TGF Beta 1)).
Product Details
- Molecular Aliases
- LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1; TGF-b-1; TGF-b1; LRRC32&TGF-b 1
- Protein Length
- Ile18-Asn628
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Rat GARP&Latent TGF Beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Rat GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.
mmobilized Human ITGAV&ITGB6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 0.4μg/ml determined by ELISA.
Background
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
References
- Stockis J, Dedobbeleer O, Lucas S. Role of GARP in the activation of latent TGF-β1. Mol Biosyst. 2017 Sep 26;13(10):1925-1935.
- Metelli A, Salem M, Wallace CH, Wu BX, Li A, Li X, Li Z. Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer. J Hematol Oncol. 2018 Feb 20;11(1):24.
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