Rat IL-13Ra2 Protein
Catalog No: IL13RA2-RF001
- Species
- Rat
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Rat IL-13Ra2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu24-Lys336 (UniProt accession: Q8VHK6).
Product Details
- Molecular Aliases
- CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13R subunit alpha-2; IL-13Ra2;IL13R alpha 2; IL13R subunit alpha-2
- Protein Length
- Leu24-Lys336
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-13, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test).
Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200).
Loaded Rat IL-13Ra2, hFc Tag on ProA-Biosensor can bind Human IL-13, His Tag with an affinity constant of 2.53 nM as determined in BLI assay.
Background
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
References
- Suzuki A, Leland P, Joshi B H, et al. Targeting of IL-4 and IL-13 receptors for cancer therapy[J]. Cytokine, 2015, 75(1):S1043466615002124. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.