Biotinylated Canine VEGF164 Protein
Catalog No: VEGF164-DB001
- Species
- Canine
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Canine VEGF164 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Ala27-Arg190 (accession: NP_001103972.1).
Product Details
- Molecular Aliases
- VEGF; VEGFA; VEGF-A; VPF
- Protein Length
- Ala27-Arg190
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 22.05 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Canine VEGF164 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Canine VEGF164, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. (QC Test)
Background
Vascular endothelial growth factor (VEGF) is an essential regulator of vascularization. It is expressed as several splice variants; the major forms contain 120 amino acids, 164 amino acids, and 188 amino acids. VEGF-A164 induces an extremely heterogeneous response that includes vascular hyperpermeability, extravascular fibrin deposition, edema, angiogenesis (mother vessels, GB, daughter capillaries), arteriovenous malformations, arteriogenesis, fibrogenesis, and lymphangiogenesis.
References
- Grunstein J, Masbad JJ, Hickey R, Giordano F, Johnson RS. Isoforms of vascular endothelial growth factor act in a coordinate fashion To recruit and expand tumor vasculature. Mol Cell Biol. 2000 Oct;20(19):7282-91. doi: 10.1128/MCB.20.19.7282-7291.2000. PMID: 10982845; PMCID: PMC86282.
- Nagy JA, Dvorak AM, Dvorak HF. VEGF-A(164/165) and PlGF: roles in angiogenesis and arteriogenesis. Trends Cardiovasc Med. 2003 Jul;13(5):169-75. doi: 10.1016/s1050-1738(03)00056-2. PMID: 12837578.
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