Human NPR1/NPRA Protein
Catalog No: NPR1-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human NPR1/NPRA Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly33-Glu473 (accession: NP_000897.3).
Product Details
- Molecular Aliases
- ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
- Protein Length
- Gly33-Glu473
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 74.56 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NPR1 is greater than 90% as determined by SEC-HPLC.
Immobilized Human NPR1, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 43.2ng/ml determined by ELISA (QC Test).
Loaded Human NPR1, mFc Tag on Anti-Mouse IgG Fc-Biosensor can bind Anti-NPR1 Antibody, hFc-Avi tag with an affinity constant of 1.20 nM as determined in BLI assay.
Background
NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
References
- Tokudome T, Otani K, Mao Y, Jensen LJ, Arai Y, Miyazaki T, Sonobe T, Pearson JT, Osaki T, Minamino N, Ishida J, Fukamizu A, Kawakami H, Onozuka D, Nishimura K, Miyazato M, Nishimura H. Endothelial Natriuretic Peptide Receptor 1 Play Crucial Role for Acute and Chronic Blood Pressure Regulation by Atrial Natriuretic Peptide. Hypertension. 2022 Jul;79(7):1409-1422. doi: 10.1161/HYPERTENSIONAHA.121.18114. Epub 2022 May 9. PMID: 35534926.
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