Mouse DNAM-1/CD226 Protein, Ultra Low Endotoxin
Catalog No: DNAM1-MP002
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse DNAM-1/CD226 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Glu19-Pro254 (UniProt accession: Q8K4F0).
Product Details
- Molecular Aliases
- CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
- Protein Length
- Glu19-Pro254
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28.39 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse DNAM-1 is greater than 95% as determined by SEC-HPLC.
Loaded Mouse DNAM-1, His Tag on Anti-His-Biosensor can bind Biotinylated Human CD155, hFc-Avi tag with an affinity constant of 9.64 nM as determined in BLI assay. (QC Test)
Background
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.