Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc
Catalog No: KRASG12D-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Biotin
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR. It contains KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Product Details
- Molecular Aliases
- KRAS G12D TCR&CD3
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 196.0 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. (QC Test)
Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.53μg/ml determined by ELISA.
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 0.47 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay.
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 2.60 nM as determined in BLI assay.
Background
The JD1a41b1 TCR is an engineered, high-affinity T-cell receptor that strictly recognizes the peptide fragment of KRAS G12D (VVVGADGVGK) presented on the HLA-A*11:01 only. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.
References
- (1) Poole, Andrew, et al. "Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigen." Nature communications 13.1 (2022): 5333. DOI: 10.1038/s41467-022-32811-1. PMID: 36088370, PMCID: PMC9464187
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