Biotinylated Human FGFR1 alpha (IIIc) Protein
Catalog No: FGFR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Arg22-Glu374 (UniProt accession: P11362-7).
Product Details
- Molecular Aliases
- FGF R1a; FGFR1 alpha
- Protein Length
- Arg22-Glu374
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-BisPAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human FGFR1 alpha (IIIc), His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)
Background
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
References
- G?Ke F, G?Ke A, Von M?Ssenhausen A, et al. Fibroblast Growth Factor Receptor 1 as a Putative Therapy Target in Colorectal Cancer[J]. Digestion, 2013, 88(3):172-181.
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