Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein
Catalog No: MAGEA2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KMVELVHFL).
Product Details
- Molecular Aliases
- MAGE-A2;MAGEA2
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Melanoma antigen gene (MAGE)-A2 (MAGEA2) is a member of the MAGE-A family proteins widely studied for cancer vaccine development and identification of tumor markers. MAGEA2 plays an oncogenic role in glioma progression, and they provide insight into MAGEA2 application as a novel predictor of clinical outcomes and a potential glioma biomarker.
References
- Meng Q, Luo G, Liu B, Sun Y, Yan Z. Melanoma-associated antigen A2 is overexpressed in glioma and associated with poor prognosis in glioma patients. Neoplasma. 2018;65(4):604-609. doi: 10.4149/neo_2018_170625N440. PMID: 30064232.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.