Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling)
Catalog No: NCR1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the N-terminus. It contains amino acid residues Gln22-Asn254 (accession: AAH64806.1).
Product Details
- Molecular Aliases
- CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094; MAR1
- Protein Length
- Gln22-Asn254
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKp46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKp46 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human NKp46, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA. (QC Test)
Background
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
References
- Glasner A, Isaacson B, Viukov S, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression.[J]. Sci Rep, 2017, 7(1):13090.
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