Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein
Catalog No: EFNA3-HF001
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln23-Ser213 (accession: P52797-1(Human) / A0A2K5VZR7(Cynomolgus) / NP_001252612.1(Rhesus macaque)).
Product Details
- Molecular Aliases
- Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3;EFL2; EHK1L
- Protein Length
- Gln23-Ser213
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus/Rhesus macaque Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque Ephrin-A3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human EPHA10, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
Background
Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.
References
- Duffy SL, Steiner KA, Tam PP, Boyd AW. Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Gene Expr Patterns. 2006 Oct;6(7):719-23. doi: 10.1016/j.modgep.2005.12.007. Epub 2006 Feb 8. PMID: 16466970.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.