Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein
Catalog No: CD3E-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, an Avi
- Activity
- Activity verified
Product overview
Recombinant Human CD3E&CD3G Protein is expressed as a heterodimer of CD3E and CD3G by co-expression in HEK293. Subunit CD3E cotains no tag and subunit CD3G is fused with a His tag and an Avi tag at the C-terminus. It contains amino acid residues Gln22-Asp126 (CD3E) and Gln23-Ser116 (CD3G) (accession: P07766(CD3E) & P09693(CD3G)).
Product Details
- Molecular Aliases
- CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g; CD3 e; CD3 g; CD3 e&CD3 g
- Protein Length
- Gln22-Asp126
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.92 kDa (CD3E) and 19.67 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD3E&CD3G, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 15.6ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human CD3E&CD3G, His-Avi tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind OKT3, mFc Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind Foralumab, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Background
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
References
- Penaranda C, Tang Q, Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.
- Gedeon P C, Schaller T H, Chitneni S K, et al. A rationally designed fully human EGFRvIII: CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma.[J]. Clinical Cancer Research, 2018:clincanres.0126.2017.
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