Human IL-18RAP/IL-18 R beta Protein
Catalog No: IL18RAP-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-18RAP/IL-18 R beta Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Phe20-Arg356 (UniProt accession: O95256-1).
Product Details
- Molecular Aliases
- IL-18 R beta; ACPL; CD218b; CDw218b; IL-18R-beta; IL-18RAcP; IL-18Rbeta; IL-1R-7; IL-1R7; IL-1RAcPL; IL18RB; IL18RAP; IL-18R-bet; IL-18R-β; IL-18Rβ;IL18R beta; IL18R-beta; IL18RAcP; IL18Rbeta; IL1R7; IL1RAcPL; IL18R-bet; IL18R-β; IL18Rβ;IL-18R-b; IL18R-b; IL-18Rb; IL-18R beta
- Protein Length
- Phe20-Arg356
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-18RAP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-18RAP is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-18RAP, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Background
lactoferrin (LTF), peptidoglycan recognition protein 1 and IL-18 receptor accessory protein (IL18RAP) remained significantly elevated in peripheral monocytes of hypertensive individuals in a separate validation cohort. Interestingly, IL18RAP expression associated with MAP in a cohort of African Americans..
References
- Hedl M, Zheng S, Abraham C. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol. 2014;192(12):5924-5932. doi:10.4049/jimmunol.1302727 Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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