Biotinylated Human FcRn Protein
Catalog No: FCRN-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human FcRn Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala24-Ser297(FCGRT)&Ile21-Met119(B2M) (accession: P55899(FCGRT) & P61769((B2M)).
Product Details
- Molecular Aliases
- FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
- Protein Length
- Ala24-Ser297
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human FcRn is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay (Biacore T200). (QC Test)
Human Serum Albumin, His Tag (Cat. BSA-HM101) immobilized on CM5 Chip can bind Biotinylated Human FcRn, His-Avi tag with an affinity constant of 16.35 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human FcRn, His-Avi tag on Streptavidin-Biosensor can bind Human Serum Albumin, Llama Fc Tag (Cat. BSA-HM501) with an affinity constant of 24.7 nM as determined in BLI assay.
Loaded Biotinylated Human FcRn, His-Avi tag on Streptavidin-Biosensor can bind Human IgG1 Fc, No Tag (Cat. IGG-HM001) with an affinity constant of 22.8 nM as determined in BLI assay.
Background
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14, and forms an MHC class I-like heterodimer.
References
- Kiessling P, Lledo-Garcia R, Watanabe S, et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study[J]. Science translational medicine, 2017, 9(414):eaan1208.
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