Biotinylated Mouse SOST/Sclerostin Protein
Catalog No: SOST-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse SOST/Sclerostin Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln24-Tyr211 (UniProt accession: Q99P68).
Product Details
- Molecular Aliases
- sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
- Protein Length
- Gln24-Tyr211
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.04 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Mouse SOST, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 13.2ng/ml determined by ELISA. (QC Test)
Background
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
References
- Claire M N, Patton D, Hayes J S. Sclerostin Antibody Therapy for the Treatment of Osteoporosis: Clinical Prospects and Challenges[J]. Journal of Osteoporosis, 2016, 2016:1-22.
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