Human IL-2 R alpha/CD25 Protein
Catalog No: CD25-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-2 R alpha/CD25 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu22-Cys213 (UniProt accession: P01589).
Product Details
- Molecular Aliases
- CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR;IL2R alpha
- Protein Length
- Glu22-Cys213
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 47.68 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. (QC Test)
Immobilized Human IL-2 R alpha, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
Background
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
References
- Smith K A. The structure of IL2 bound to the three chains of the IL2 receptor and how signaling occurs[J]. Medical Immunology, 2006, 5(1).
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