Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein
Catalog No: CD32BC-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Fc gamma RIIB/C (CD32b/c) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala46-Pro217 (UniProt accession: P31994-1).
Product Details
- Molecular Aliases
- Fc gamma RII; Fc gamma RIIB; Fc gamma RIIC; CD32; CD32b; CD32c; CDw32; FCG2; IGFR2; CD32A; FCG2; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB
- Protein Length
- Ala46-Pro217
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIB/C, His-Avi tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 11.41 μM as determined in SPR assay (Biacore S200). (QC Test)
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIB/C, His-Avi tag with an affinity constant of 3.97 μM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human Fc gamma RIIB/C, His-Avi tag on Streptavidin-Biosensor can bind Rituximab with an affinity constant of 2.40 µM as determined in BLI assay.
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
References
- Engelhardt W, Geerds C, Frey, Jürgen. DISTRIBUTION, INDUCIBILITY AND BIOLOGICAL FUNCTION OF THE CLONED AND EXPRESSED HUMAN BETA-FC RECEPTOR-II[J]. European Journal of Immunology, 2010, 20(6):1367-1377.
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