Human FGFR2 alpha (IIIc) Protein
Catalog No: FGFR2-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Human FGFR2 alpha (IIIc) Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Arg22-Glu377 (UniProt accession: P21802-1).
Product Details
- Molecular Aliases
- FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662
- Protein Length
- Arg22-Glu377
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGFR2 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FGFR2 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.
Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
References
- D'Amici S, Ceccarelli S, Vescarelli E, Romano F, Frati L, Marchese C, Angeloni A. TNFα modulates Fibroblast Growth Factor Receptor 2 gene expression through the pRB/E2F1 pathway: identification of a non-canonical E2F binding motif. PLoS One. 2013 Apr 16;8(4):e61491. doi: 10.1371/journal.pone.0061491. PMID: 23613863; PMCID: PMC3629046.
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