Mouse Fc gamma RI/CD64 Protein
Catalog No: CD64-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse Fc gamma RI/CD64 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Glu25-Pro297 (UniProt accession: P26151-1).
Product Details
- Molecular Aliases
- CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI; FcγRI; Fc γRI; Fcγ RI; Fc γ RI
- Protein Length
- Glu25-Pro297
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RI is greater than 95% as determined by SEC-HPLC.
Loaded Trastuzumab, hFc Tag on ProA-Biosensor can bind Mouse Fc gamma RI, His Tag with an affinity constant of 56.70 nM as determined in BLI assay. (QC Test)
Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200).
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
References
- Nimmerjahn F, Ravetch J V. Fcγ receptors as regulators of immune responses[J]. Nature Reviews Immunology, 2008, 8(1):34-47.
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