Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Tetramer Protein
Catalog No: MART1-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ELAGIGILTV).
Product Details
- Molecular Aliases
- MART1; LB39-AA; SK29-AA; Melan-A; MLANA
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Tetramer is greater than 95% as determined by SEC-HPLC.
Background
Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
References
- Wang J, Jia R, Yao Y, Cun B, Huang X, Gu P, Ge S, Fan X. Differential expression of Mart-1 in human uveal melanoma cells. Mol Med Rep. 2011 Sep-Oct;4(5):799-803. doi: 10.3892/mmr.2011.504. Epub 2011 Jun 7. PMID: 21667026.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.