Biotinylated Human HLA-A*02:01&B2M&RAB7B (KLIIVGAIGV) Monomer Protein
Catalog No: HLAA0201-HB001
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&RAB7B (KLIIVGAIGV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KLIIVGAIGV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KLIIVGAIGV).
Product Details
- Molecular Aliases
- MHC; RAB7B
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCI, 10% glycerol (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*02:01&B2M&RAB7B (KLIIVGAIGV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&RAB7B (KLIIVGAIGV) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Rab proteins are key-regulators of intracellular membrane trafficking. Rab7b is a recently identified Rab protein that may downregulate TLR4 and TLR9-mediated inflammatory responses. Rab7b, believed to have similar function as Rab7, controls however vesicular trafficking from endosomes to the TGN. It is localized to late endosomes/lysosomes as well as the TGN. Rab7b interferes with enzymes delivery to lysosomes and with the retrograde Shiga toxin transport to the Golgi.
References
- Bucci C, Bakke O, Progida C. Rab7b and receptors trafficking. Commun Integr Biol. 2010 Sep;3(5):401-4. doi: 10.4161/cib.3.5.12341. PMID: 21057625; PMCID: PMC2974065.
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