PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein
Catalog No: PRAME-HP007
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLLQHLIGL).
Product Details
- Molecular Aliases
- PRAME; OIP-4; OIP4; MAPE;
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl, 0.2% BSA (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HE045TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. (QC Test)
Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
Background
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
References
- Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.
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