Human CD28H/IGPR-1 Protein
Catalog No: CD28H-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD28H/IGPR-1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu23-Gly150 (UniProt accession: Q96BF3-1).
Product Details
- Molecular Aliases
- TMIGD2; IGPR1; IGPR-1; CD28H; TPC1
- Protein Length
- Leu23-Gly150
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD28H on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.
Immobilized Human B7-H7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 63.4ng/ml determined by ELISA (QC Test).
Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human B7-H7, His-Avi tag on Streptavidin-Biosensor can bind Human CD28H, hFc Tag with an affinity constant of 5.28 nM as determined in BLI assay.
Background
CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
References
- Zhu Y, Yao S, Iliopoulou BP, Han X, Augustine MM, Xu H, Phennicie RT, Flies SJ, Broadwater M, Ruff W, Taube JM, Zheng L, Luo L, Zhu G, Chen J, Chen L. B7-H5 costimulates human T cells via CD28H. Nat Commun. 2013;4:2043. doi: 10.1038/ncomms3043. PMID: 23784006; PMCID: PMC3698612. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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