Biotinylated Human PD-L1/B7-H1 Protein
Catalog No: PDL1-HB006
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human PD-L1/B7-H1 is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Phe19-Arg238 (UniProt accession: Q9NZQ7-1).
Product Details
- Molecular Aliases
- CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
- Protein Length
- Phe19-Arg238
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human PD-L1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human PD-1, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 9.4ng/ml determined by ELISA.
Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi tag binding reactions. The half maximal inhibitiory concentration (IC50) is 0.22μg/ml.
Loaded Biotinylated Human PD-L1, His Tag on Streptavidin-Biosensor can bind Human PD-1, His Tag with an affinity constant of 0.94 µM as determined in BLI assay.
Background
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
References
- Blank C, Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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