Human Nectin-4 Protein, Ultra Low Endotoxin
Catalog No: NECTIN4-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human Nectin-4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly32-Ser349 (UniProt accession: Q96NY8-1).
Product Details
- Molecular Aliases
- EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
- Protein Length
- Gly32-Ser349
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Nectin-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1μg/ml determined by ELISA (QC Test).
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Background
NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.
References
- Chatterjee S, Sinha S, Kundu CN. Nectin cell adhesion molecule-4 (NECTIN-4): A potential target for cancer therapy. Eur J Pharmacol. 2021 Nov 15;911:174516. doi: 10.1016/j.ejphar.2021.174516. Epub 2021 Sep 20. PMID: 34547246.
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