Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein
Catalog No: CD16A-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly17-Gln208 (V176) (accession: AAH17865).
Product Details
- Molecular Aliases
- Fc gamma RIIIA; Fc gamma RIIIA (V176); FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA
- Protein Length
- Gly17-Gln208
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Fc gamma RIIIA (V176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176), His Tag with the EC50 of 9.8 ng/ml determined by ELISA (QC Test).
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIIA (V176), His-Avi tag with an affinity constant of 0.539 μM as determined in SPR assay (Biacore T200).
Background
The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
References
- Koene H R, Kleijer M, Algra J, et al. Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype[J]. Blood, 1997, 90(3):1109-1114.
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