Biotinylated Human SIRP gamma/CD172g Protein
Catalog No: SIRPG-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu29-Pro360 (UniProt accession: Q9P1W8-1).
Product Details
- Molecular Aliases
- CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2; SIRPγ; SIRP γ
- Protein Length
- Glu29-Pro360
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human SIRP gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Biotinylated Human SIRP gamma is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human SIRP Gamma, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.28μg/ml determined by ELISA.
Background
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
References
- Piccio, L. Adhesion of human T cells to antigen-presenting cells through SIRP beta2-CD47 interaction costimulates T-cell proliferation[J]. Blood, 2005, 105(6):2421-2427. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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