FITC-Labeled Human B7-H3 (4Ig) /B7-H3b Protein
Catalog No: B7H3-HP009
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant FITC-Labeled Human B7-H3 (4Ig) /B7-H3b Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly27-Thr461 (UniProt accession: Q5ZPR3-1).
Product Details
- Molecular Aliases
- B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
- Protein Length
- Gly27-Thr461
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
FITC-Labeled Human B7-H3 (4Ig) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.
Immobilized FITC-Labeled Human B7-H3 (4Ig), hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.5ng/ml determined by ELISA.
Background
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
References
- Fodstad O, Tekle C, Chen Y W. The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5) Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.