Biotinylated Human B7-H3 (2Ig)/CD276 Protein
Catalog No: B7H3-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Leu29-Pro245 (UniProt accession: Q5ZPR3-2).
Product Details
- Molecular Aliases
- B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276
- Protein Length
- Leu29-Pro245
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51.06 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-B7-H3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H3, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human B7-H3, hFc-Avi tag on Streptavidin-Biosensor can bind Anti-B7-H3 Ab., hFc Tag with an affinity constant of 0.30 nM as determined in BLI assay.
Background
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
References
- Fodstad O, Tekle C, Chen Y W. The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5)
- Du H, Hirabayashi K, Ahn S, et al. Antitumor Responses in the Absence of Toxicity in Solid Tumors by Targeting B7-H3 via Chimeric Antigen Receptor T Cells[J]. Cancer Cell, 2019, 35(2). Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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