Biotinylated Human/Cynomolgus Activin RIIA/ACVR2A Protein
Catalog No: ACTIVINRIIA-HB003
- Species
- Human/Cyno
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human/Cynomolgus Activin RIIA/ACVR2A Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala20-Pro135 (accession: P27037-1(Human) / G7PKJ4(Cynomolgus)).
Product Details
- Molecular Aliases
- ACTR-IIA; ACTRIIA; ACVR2A; ACVR2
- Protein Length
- Ala20-Pro135
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.31 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Cynomolgus Activin RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human/Cynomolgus Activin RIIA is greater than 95% as determined by SEC-HPLC.
Immobilized Human/Mouse/Rat Activin A, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIA, His Tag with the EC50 of 0.23 μg/ml determined by ELISA. (QC Test)
Biotinylated Human/Cynomolgus Activin RIIA, His Tag immobilized on CM5 Chip can bind Human Activin A, No Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Background
Activin A receptor type 2A (ACVR2A) is a membrane receptor in the transforming growth factor- beta (TGF-β signaling pathway, which is involved in the regulation of cell proliferation, migration, and apoptosis. Loss of ACVR2A has an important role in cancer progression and distant metastasis and may serve as a prognostic marker in patients with colon cancer.
References
- Zhuo C, Hu D, Li J, Yu H, Lin X, Chen Y, Zhuang Y, Li Q, Zheng X, Yang C. Downregulation of Activin A Receptor Type 2A Is Associated with Metastatic Potential and Poor Prognosis of Colon Cancer. J Cancer. 2018 Sep 8;9(19):3626-3633. doi: 10.7150/jca.26790. PMID: 30310521; PMCID: PMC6171025. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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