Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Monomer Protein, Ultra Low Endotoxin
Catalog No: SURVIVIN2B-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human Survivin 2B(HLA-A*24:02) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and AYACNTSTL peptide (accession: AAA59600.1(HLA-A*24:02) & P61769(B2M) &AYACNTSTL).
Product Details
- Molecular Aliases
- svn-2B; Survivin-2B; svn 2B
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*24:02&B2M&Survivin 2B (AYACNTSTL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Survivin-2B, a known splice variant of survivin, has been reported to promote cell death in some cancer cells, although it keeps prosurvival function in others.survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus.
References
- Shi K, An J, Shan L, Jiang Q, Li F, Ci Y, Wu P, Duan J, Hui K, Yang Y, Xu C. Survivin-2B promotes autophagy by accumulating IKK alpha in the nucleus of selenite-treated NB4 cells. Cell Death Dis. 2014 Feb 20;5(2):e1071. doi: 10.1038/cddis.2014.34. PMID: 24556686; PMCID: PMC3944251.
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