Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein
Catalog No: CMVPP65-HP004
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &NLVPMVATV).
Product Details
- Molecular Aliases
- CMVpp65; NLVPMVATV
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reduced (N) condition.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer is greater than 95% as determined by SEC-HPLC.
Background
Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
References
- [1] Lee SY, Ko DH, Son MJ, Kim JA, Jung K, Kim YS. Affinity Maturation of a T-Cell Receptor-Like Antibody Specific for a Cytomegalovirus pp65-Derived Peptide Presented by HLA-A*02:01. Int J Mol Sci. 2021 Feb 26;22(5):2349. doi: 10.3390/ijms22052349.
- [2] Hasan AN, Doubrovina E, Sottile R, Prockop S, Klatt MG, Heller G, Selvakumar A, Barnett L, Hsu KC, O'Reilly RJ. Dominant epitopes presented by prevalent HLA alleles permit wide use of banked CMVpp65 T cells in adoptive therapy. Blood Adv. 2022 Aug 23;6(16):4859-4872. doi: 10.1182/bloodadvances.2022007005.
- [3] Zaia JA, Gallez-Hawkins G, Li X, Yao ZQ, Lomeli N, Molinder K, La Rosa C, Diamond DJ. Infrequent occurrence of natural mutations in the pp65(495-503) epitope sequence presented by the HLA A*0201 allele among human cytomegalovirus isolates. J Virol. 2001 Mar;75(5):2472-4. doi: 10.1128/JVI.75.5.2472-2474.2001.
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