Non-biotinylated Human FGF21 Protein
Catalog No: FGF21-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Human FGF21 Protein is expressed in HEK293 cells with an mFc (IgG1) tag and Avi tag at the N-terminus. It contains amino acid residues His29-Ser209 (UniProt accession: Q9NSA1-1).
Product Details
- Molecular Aliases
- UNQ3115; PRO10196; FGF-21; FGF21
- Protein Length
- His29-Ser209
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGF21 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag (Cat. KLB-HM101) with the EC50 of 0.75μg/ml determined by ELISA (QC Test).
Human Beta Klotho, His Tag (Cat. KLB-HM101) captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Loaded Human Beta Klotho, His Tag (Cat. KLB-HM101) on Aint-His-Biosensor can bind Human FGF21, mFc Tag with an affinity constant of 0.16 nM as determined in BLI assay.
Background
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
References
- Lewis JE, Ebling FJP, Samms RJ, Tsintzas K. Going Back to the Biology of FGF21: New Insights. Trends Endocrinol Metab. 2019;30(8):491-504. doi:10.1016/j.tem.2019.05.007
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