Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein
Catalog No: GP100-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &YLEPGPVTA).
Product Details
- Molecular Aliases
- HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His-Avi tag with the EC50 of 21.7ng/ml determined by ELISA.
Background
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I and YLEPGPVTA peptide of the gp100.
References
- (1)Abad J D, Wrzensinski C, Overwijk W, et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.
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